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ELAB (Enantiomerlabeling): One of the Most Potent Amino Acid Analysis Methods

METHOD: The use of D-amino acids as an ideal multi internal standard

  
By capillary gas chromatography on a chiral stationary phase it is possible to separate the amino acids together with their enantiomers, thus allowing the determination of enantiomeric purity after hydrolysis. In a second analytical run the sample is spiked with an aliquot of the optical antipodes of the amino acids and d-norvaline as standard for glycine. These serve as ideal multi internal standards which are calibrated against certified standards from National Institute of Standards and Technology (NIST).  

General information: ELAB.pdf

Standard deviation: AAA_Std.pdf

Detailed information about enantiomerlabeling: elab-publication.pdf

 

Advantages:

  • The advantages of this method of analysis may be summarized as follows:
  • High accuracy of the analysis even after extensive sample preparation. Also Serine, Tyrosine and Tryptophan is determined after acid hydrolysis with high accuracy.
  • In addition to quantitation of the amino acids, information of the optical purity of the sample might be provided.
  • Possibility of using a large number of methods for sample preparation without affecting the accuracy of the result.
  • The multitude of GC-detectors may be used, including MS coupling.
  
Order A400: AAA with determination of blind values (enantiomeric purity after hydrolysis)  
Order A420: AAA without determination of blind values (not for GMP products)  
Order A460: Loading of resin bond amino acids   
 

 

 
 
     

   © 2004 by Daniel Gerhardt •  daniel.gerhardt@cat-online.com