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GC-MS method |
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| The unambiguous quantitation of
racemate involves hydrolysis with 6N D2O/DCl. The amino acids are
derivatized using deuterated reagents. Racemization during this sample
preparation is accompanied by deuterium exchange in the a-position (deuterium
label). The proportion of D-amino acid originally present in the peptide is
thus represent by the relative amounts of the unlabeled form which is
monitored by mass spectrometry. The limit of quantitation is 0.1% of the optical antipode. The standard deviation is <0.1%. |
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| What we need: 0.5 to 1mg of the sample (for LOQ of 0.1% of the enantiomer). The amino acids which are to determine or better the sequence. Protective groups if present. Expected content. |
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© 2004 designed by Daniel Gerhardt • daniel.gerhardt@cat-online.com