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Determination of other amino acids in amino acids and amino acid derivatives
We offer three different methods for determination of other amino acids in amino acids and amino acid derivatives . All methods are possible only in combination with e.g.. determination of optical purity:   
Semi quantitative determination (A445)
 
Quantitative determination (A470)  
Quantitative determination via GC-MS (A785)  
 For free amino acids:
A445 can be mostly used for free amino acids if
  • LOQ of 0.3% is sufficient and
  • Histidine needs not to be determined
  • In addition it is possible that Cys, Met and Trp can be discriminated without any indication.

 If A455 is applicable can be said after determination of enantiomeric purity.

  
 For amino acid derivatives:
The situation for amino acid derivative is different, because matrix effects and contaminations are observed more often. During the course of A470 these problems are mostly indicated and are possibly to overcome. If A455 is applicable for derivatives too can be said after determination of enantiomeric purity.		  
 For peptides:
 For small peptides in high purity the argumentation is similar to the amino acid derivatives. In the case of longer peptides and samples which shows some contaminations decomposition products or other contaminations even in low concentration can co-elute with amino acids which are to determine as traces. The results are falsify. In these cases A785 can give most reliable results due to the high selectivity.

 

 

 

 
 
     

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