Determination of peptide sequence via Edman degradation
Cyclic degradation of peptides based on the reaction of
phenylisothiocyanate with the free amino group of the N-terminal residue such
that amino acids are removed one at a time and identified as their
phenylthiohydantoin derivatives by HPLC:
Results:
Concentration of the amino acids in each degradation cycle
What we need:
<1mg of the sample.
(The sequence of the peptide)
Protective groups if present.
Expected content.