We offer one of the most efficient amino acid analysis,the Enantiomerlabeling: The amino acid enantiomers are used as excellent multi-internal standards.

For the calculation the enantiomeric purity of the standard as well of the sample is taken into account.

Calculation of the amino acid concentration:

A_L = peak area of L enantiomer after addition of standard
A_D = peak area of D enantiomer after addition of standard
C_L = D enantiomer : L enantiomer of the sample
C_D = L enantiomer : D enantiomer of the standard amino acid
M_D = amount of standard amino acid added
X_A = amount of amino acid being determined

Two methods are available:

with determination of blind values:
The enantiomeric purity of the amino acids after! hydrolysis is determined.

without determination of blind values:
the peptide is assumed to have the correct configuration for all amino acids and no contaminants by the wrong enantiomers. Furthermore it is assumed that no substance specific racemization take place during hydrolysis. Therefore this method is not capable for GMP-products.

• For more details download PDF Publication.
• For more details download PDF Introduction.