The second unambiguous quantitation of racemate involves hydrolysis with 6N D₂O/DCl. The amino acids are derivatized using deuterated reagents. Racemization during this sample preparation is accompanied by deuterion exchange in the a-position (deuterium label). The original proportion of D-amino acid in the peptide, is represented by the relative amounts of the unlabeled form which is monitored by mass spectrometry. The limit of quantitation is 0.1% of the optical antipode. The standard deviation is <0.1%

Results: Calculated optical purity of amino acids

Reaction pathway

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