For sequencing we offer usually MS/MS sequencing. It depends on the sequence weather a complete verification is possible or not. For peptides having 20-30 residues of amino acids complete verification is mostly possible.

Difference between Edman and MSn sequencing:

Whereas Edman degradation starts at the N-terminus MSn fragments the complete molecule. In consequence

Edman might fail under circumstances after a couple of amino acids whereas

MS/MS might fail for longer peptides at the N- and/or C-terminus.

In contrast to Edman MS/MS might fail only for a short part of the sequence but verifies the sequence on both sides of the unverified part.

• For information about limitations of the methods download PDF.